A subset of melanoma patients has evidence for sponta-neous anti-tumor immune responses and T cell infiltration into tumor sites, which has important prognostic value and is associated with clinical responses to immunothera-pies. However, the molecular mechanisms explaining absence of a T cell response in the majority of patients are not defined. Analyses of human melanoma metastases by exome sequencing, gene expression profiling, and IHC have revealed that many tumors that lack a T cell signa-ture show alterations in the Wnt/b-catenin signaling path-way. To investigate if constituently active b-catenin signaling within the tumor cells might inhibit immune responses, we utilized an inducible autochthonous mouse melanoma model driven by inducible Braf V600E and PTEN-deletion, with or without inducible expression of active b-catenin. While Braf/PTEN melanomas showed presence of a modest T cell infiltrate, T cells were nearly completely eliminated in tumors expressing active b-catenin. The T cells that were present in the Braf/PTEN tumors showed an exhausted phenotype as what has been seen in transplanted tumor models, and therapeutic efficacy of combination therapy using aCTLA4 with aPD-L1 mAbs was limited to Braf/PTEN tumors. To test whether the lack of T cell infiltration was due to a defect in early T cell priming, these mice were additionally bred to inducibly express the SIY model antigen within the developing tumors. Adoptive transfer of 2C TCR Tg SIY-specific T cells revealed defective spontaneous T cell priming when tumors expressed active b-catenin. Analysis of the antigen-presenting cell compartment revealed a selective decrease in the CD103 + DC subset within the tumor microenvironment, and T cell infiltration could be restored by intra-tumoral injection of FLT3-ligand-derived dendritic cells. The lack of CD103 + dermal dendritic cells was associated with reduced expression of the chemokines CCL4 and CXCL1. Surprisingly we identified that tumor cells themselves as the major chemokine source in Braf/ PTEN tumors, while tumors with active b-catenin signal-ing lacked expression of those chemokines. Therefore, our data have identified the first defined molecular pathway in tumor cells that results in defective spontaneous anti-tumor T cell responses, an observation with important implications for cancer immunotherapy.
CITATION STYLE
Spranger, S., Bao, R., & Gajewski, T. (2014). Melanoma-intrinsic β-catenin signaling prevents T cell infiltration and anti-tumor immunity. Journal for ImmunoTherapy of Cancer, 2(S3). https://doi.org/10.1186/2051-1426-2-s3-o15
Mendeley helps you to discover research relevant for your work.