Mass and growth rate are highly integrative measures of cell physiology not discernable via genomic measurements. Here, we introduce a microfluidic platform enabling direct measurement of single-cell mass and growth rate upstream of highly multiplexed single-cell profiling such as single-cell RNA sequencing. We resolve transcriptional signatures associated with single-cell mass and growth rate in L1210 and FL5.12 cell lines and activated CD8+ T cells. Further, we demonstrate a framework using these linked measurements to characterize biophysical heterogeneity in a patient-derived glioblastoma cell line with and without drug treatment. Our results highlight the value of coupled phenotypic metrics in guiding single-cell genomics.
CITATION STYLE
Kimmerling, R. J., Prakadan, S. M., Gupta, A. J., Calistri, N. L., Stevens, M. M., Olcum, S., … Manalis, S. R. (2018). Linking single-cell measurements of mass, growth rate, and gene expression 06 Biological Sciences 0604 Genetics. Genome Biology, 19(1). https://doi.org/10.1186/s13059-018-1576-0
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