New stigmastane steroids constituents from rice hulls of Oryza sativa and inhibitory activity on radish seed

Abstract

Two new compounds 1'-(stigmast-11,20(21),25-trien-3α,9α-diolyl) -3′-(pimara-11,15-dien-3α-olyl) glycerol (1) and stigmast-5-en-3α,26-diol (2) along with known fatty acids n-hexacosanoic acid (3) and hexadecanoic acid (4), have been isolated from the methanol extract of rice hulls of Oryza sativa. The structures of the compounds were elucidated using 1D and 2D NMR spectral methods, viz; 1H, 13C, 13C-DEPT, 1H-1H COSY, 1H- 13C HETCOR, HSQC and HMBC aided by IR, EIMS, FABMS and HRFABMS. Compound (1) showed inhibition to radish germination, growth of shoot and root length.