Prevalence of resistant Salmonella spp. isolated from raw meat and liver of cattle in the Wa Municipality of Ghana

Abstract

This study was carried out to determine the prevalence of resistant Salmonella spp. isolated from meat (beef) and liver of cattle in the Wa, Municipality of Ghana. Isolation of Salmonella spp. was done according to the USA-FDA Bacteriological Analytical Manual. Antibiotic susceptibility test was performed using the disc diffusion method and the results interpreted using the CLSI guidelines. Antibiotic residues determination was carried out using Premi®Test Kit. Liver samples 32%(16/50) were more contaminated with Salmonella spp. as compared to beef samples 30% (15/50). The Salmonella spp. were highly resistant to teicoplanin (96.77%) but susceptible to chloramphenicol (100%), ciprofloxacin (100%), tetracycline (100%), suphamethoxazole/trimethoprim (100%), amoxycillin/clavulanic acid (93.55%), ceftriaxone (93.55%) and gentamicin (83.87%). The Salmonella spp. exhibited 5 antibiotic resistant patterns with the pattern (Tec) being the commonest. Multiple antibiotic index ranged from 0.11 to 0.33. A total of 35 samples (20 beef and 15 liver samples) were examined for antibiotic residues, and 7 (20%) were positive for antibiotic residues; 6 (17%) were found in the liver while 1 (3%) was in the beef. This study revealed that salmonellae are present in beef and liver samples examined and showed high resistance to teicoplanin. Some of the beef and kidney samples harbored antibiotic residues.