Rapid, high-throughput library preparation for next-generation sequencing

Abstract

Advances in next-generation sequencing have led to new library preparation methods compatible with multiple sequencing platforms. Current methods (both mechanical and enzymatic) face limitations: multi-step protocols, sample loss, lack of automation and labor costs. With the continued decline of sequencing costs and increase in sample throughput, there is greater demand for more cost-effective, streamlined library preparation methods. Here we describe Epicentre's patented Nextera™ technology, which addresses current issues in sample preparation and provides a simplified procedure amenable to high-throughput workflows.