Nutritional features of Syntrophomonas wolfei

Abstract

Syntrophomonas wolfei subsp. wolfei grew poorly in a defined medium with cortonate as the energy source in the absence of rumen fluid. Thiamine, lipoic acid, biotin, cyanocobalamin, and para-aminobenzoic acid were required for growth comparable to that obtained with the rumen fluid-based medium. Iron and cobalt were also required for the growth of S. wolfei in the chemically defined medium.