Optimization of Single Cell Protein (SCP) Production using Saccharomyces Cerevisiae by Response Surface Methodology (RSM) on Cell Biomass and Protein Percentage

Abstract

This study aims to determine the effect of using molasses, KH2PO4 and MgSO4 media with different concentrations and formulations on S. cerevisiae's growth in manufacturing single-cell protein (SCP). The method used was the response surface methodology (RSM), consisting of 14 treatment formulations and 2 replications. The test parameters carried out in this study were cell biomass and crude protein content. Obtaining data were analyzed using the expert design program 11 with the response surface methodology (RSM) method and the box-Behnken experimental design. The relationship between fixed variables and independent variables of cell biomass is modeled Y:+6.54-28.63X1-0.0050X2-.0112X30.4025X1X2+0.0900X1X3-0.0775X2X3, while crude protein is modeled Y:+6.54-28.63X1-0.0050X2-0.0112X30.4025X1X2+0.0900X1X3-0.0775X2X3. The results showed that the higher the concentration of molasses, the increased cell biomass. The highest cell biomass value was obtained in the addition of 20% molasses to produce 2.33 grams, while the addition of 20% molasses and 0.7% KH2PO4 crude protein produced a low crude protein of 6.00%.