Laccase stabilization by covalent binding immobilization on activated polyvinyl alcohol carrier

Abstract

Aim: Attempts were made to immobilize laccase from Panus conchatus. Methods and Results: The laccase was immobilized on carboxylated polyvinyl alcohol (PVA) activated by N-hydroxysuccinimide (N-HSI) in aqueous solution at different pHs, temperatures, and with different reaction times. An optimum condition for laccase immobilization is at pH 3.2, 40 °C and 12 h, respectively. Immobilization of laccase increased optimal pH for reaction with 2, 2′-azinobis (3-ethylbenzthiazoline-6-solfonate) (ABTS) and pH stability. Immobilized laccase proved to be reacted consecutively 17 times with only a 50% decrease on activity and be used in removal of 2,4,6-trichlorophenol (TCP). Conclusions: It was possible to immobilize the laccase on carboxylated polyvinyl alcohol by activation with N-hydroxysuccinimide in HAc-NaAc buffer. The immobilized laccase is both stable and reusable. Significance and Impact of the Study: The results indicate that this immobilized laccase can be used in the removal of poisonous effluent from pulp bleaching mills. © 2002 The Society for Applied Microbiology.