Using the Possibilities of in Vitro Culture for the Preparation of Dietary Supplements from Alfalfa Tissues

Abstract

In vitro methods have environmental advantages of preparing bio additives as harmful chemicals such as fertilizers, herbicides, pesticides are used. In addition, obtaining biomass does not depend on seasonality and a long growing season. In this series of experiments, an accelerated method for obtaining a sterile alfalfa culture was developed by adding 1% potassium humate to the explant culture. From 4 varieties of alfalfa, 3 cell lines were selected, characterized by heterogeneity of callus tissues. 1 - line morphogenic structures; 2 - line - without meristematic foci and brown and dark brown in color, which were not further differentiated; 3- line forming polymerogenic tissues. In addition, it was possible to induce denser tissues from loose callus by passaging on the Risting medium in the Ray khan variety. Structured tissues with meristematic foci induced on Risting medium were lyophilized and the dried biomass was prepared for further biochemical analyses. Thus, we have optimized the conditions for obtaining biomass from alfalfa culture and carried out the selection of cell lines, and we assume that callus lines, upon receipt of positive biochemical analyzes, can be used as feed additives.