Gene Transfection for Stem Cell Therapy

Abstract

Genetic engineering of stem cells is a strategy that holds promise for realizing the potential therapeutic benefits of stem cell therapy. Through precise control of the stem cell genome, stem cells can replace or repair damaged tissues as well as serve as a depot for the sustained delivery of therapeutic molecules. Various individual genes, genome editing techniques, and transfection agents have been studied and developed for use in stem cell gene transfection. The goal for this review is to introduce specific genes and editing techniques used in stem cell therapy. Diverse gene transfection agents such as liposomes, polymers, dendrimers, peptides, inorganic nanoparticles, and physical transfection agents are also discussed with particular focus on stem cell considerations.