Stokes polarization imaging applied for monitoring dynamic tissue optical clearing

Abstract

We propose a continuous Stokes imaging system with a refresh rate of several seconds, instead of a traditional Mueller measurement setup, to quickly track the microstructural changes of tissues during the optical clearing process. The effectiveness of this fast Stokes imaging applied in monitoring the dynamic process is first validated by three designed experiments with a polarization state that changes continuously and rapidly, and is further confirmed by gradual changes in polarization image contrast and resolution with clearing. By comparison with experiments from different tissue samples with the same agent, the fast Stokes response curve can improve the analysis ability of photon polarization behavior connected with the complicated changes of tissue characteristics.