Disinfection of Extracted Teeth for Den-tal Researches

Abstract

Aims: To determine the effectiveness of different disinfection methods on extracted human teeth using five types of bacteria Proteus species, Escherichia coli, Kelebsiella species, Staphylococcus aureus, Streptococcus mutans. Materials and Methods: In this study extracted non-carious teeth were divided into five groups according to the type of bacteria that were inoculated inside the pulp chambers. Each group of the teeth were subdivided into six groups; group A: teeth were immersed in 2.5% NaOCl for 1week, group B: teeth were immersed in 1% NaOCl for 1week. Group C: teeth were autoclaved at 121C for at 15 Ibs psi for 15 minutes, group D: teeth disinfected using microwave for 3 minutes, group E: teeth disinfected using microwave for 6 minutes, group F: control group in which the teeth im- mersed in normal saline for seven days at room temperature. Each tooth aseptically placed in individual test tube with growth media. Simples were examined after 24h. Results: showed that autoclave, mi- crowave (when used at sex and three minutes), sodium hypochlorite at 2.5% prevented the growth completely in all types of the bacteria that were used to infect the teeth involved in this study. Key words: Disinfection, microwave, extracted teeth