Isolating Bioactive Compound from Marine Prosobranch Purpura persica from Tuticorin Coast

Abstract

Antibiotics have revolutionized life saving medicine by providing cure for many number of life threatening diseases in human history, unexpectedly, many pathogenic microorganisms have developed resistant towards current antibiotic and this trend has become more and more serious. Hence the present study has been aimed to find out new marine derived antibiotic from Prosobranch mollusc Purpura persica. The whole body crude extract of methanol was partially purified by normal phase silica gel 160-120 mesh (Glaxo, Bombay) column chromatography with low polar to high polar solvent Hexane: Chloroform (F1); Chloroform (F2); Benzene (F3); Benzene: Methanol (F4), and Methanol F5). The antimicrobial activity of crude and eluted fractions were assayed against ten bacterial pathogens viz Aeromonas hydrophila, Bacillus cereus, Escherichia coli, Pseudomonas aerogenosa, Salmnella typhi, Shigella flexneri, Vibrio cholera 0139, Vibrio cholera classical, Vibrio cholerae 01790 and Vibrio cholerae EITOR and nine fungal pathogens viz. Aspergillus flavus, Aspergillus terreus, Aspergillus niger, Aspergillus fumigatus, Fusarium moniliforme, Trichoderma sp. Penicillium citrinum, Penicillium oxallicum and Rhizopus sp. respectively using the agar disc diffusion method. To find out the most probable antibiotic compound HPLC and GC-MS studies were carried out. Among the tested bacterial pathogens S. typhi, P. aerogenosa, S. flexneri and B. cereus and fungal pathogens A. fumigatus, A. terreus, F. moniliforme and Trichoderma sp. showed inhibition in growth by crude, F2, F3 and F5 fractions of P. persica respectively. The GC-MS and HPLC analysis revealed the presence chloridate cholest-5-en- 3-01(3a) - carbano chloridate, a chloride compound 9, 12-octadecadienoyn chloride (z, z), and a cholest-5-en-3-ol(3)- carbonochloridate, eugenol, dibutyl phthalate, 1,2-Benzenedicarboxylic acid, diisooctyl ester and Phthalic acid, bis (7- methyloctyl) ester, and a steroid cholest-5-ene, 3-bromo-(3a), 2-piperidinone a monoterpene azulene, a fluro compound acetic acid, tri fluro- tetradecyl which were responsible for inhibiting the growth of microbes tested and the present test organism P. persica have great potential for developing useful drugs.