Mapping of mycoplasma virus DNA replication origins and termini

Abstract

A pulse-labeling protocol has been used to study DNA replication and map replication origins and termini in mycoplasma viruses L2 and L2ins1. The L2 genome is circular, double-stranded DNA of 11.63 kilobase pairs (kb), and the 14.89-kb L2ins1 genome is L2 DNA containing a 3.26-kb insertion. The data show that DNA replication is bidirectional from two origins in L2 and three origins in L2ins1. The extra origin in L2ins1 arises from the fact that one of the L2 origins is in one of the sequences that have been shown to be duplicated and transposed in the generation of L2ins1 from L2.