A sensitive analytical method for six aflatoxins in rainbow trout muscle and liver

Abstract

A highly sensitive analysis method for six aflatoxins (aflatoxin B 1, B 2, G 1, G 2, M 1 and aflatoxicol) in rainbow trout muscle and liver was developed. Aflatoxins (AFs) were extracted with acetonitrile-water (9 : 1), purified on an immunoaffinity column, and subjected to HPLC with fluorescence detection after post-column photochemical derivatization. The recoveries of AFs at 0.05 μg/kg spiking levels were 71.4-82.4% in muscleand 80.1-93.0% in liver, and the repeatability relative standard deviations (RSDr) were 0.87-4.6% in muscle and 2.0-6.2% in liver. Limits of quantitation (LOQs) and limits of detection (LODs) of AFs were estimated to be 0.004-0.029 μg/kg, and 0.002-0.012 μg/kg, respectively.