Optimization of phytase production in solid state fermentation by different fungi

Abstract

The present study was conducted to explore the potential fungal isolates for max. prodn. of phytase by optimizing solid substrate preference and growth parameters (pH, temp., fermn. time) of solid state fermn. (SSF). It has been inferred from the results that all the tested fungi (Aspergillus niger, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Cladosporium cladosporioides, Trichoderma reesei, and Trichoderma viride) showed inducible expression of phytase. Fungal isolates showed different preference of solid substrate out of wheat bran, lentil, oat, corn and bagasse for max. prodn. of phytase. Lentil and bagasse were not used preferably by any tested fungi. Among the tested fungi, max. phytase prodn. was obsd. in A. flavus (80 U/g of solid substrate) using wheat bran as solid substrate at pH 6 after 7 days of fermn. period at 30°C. It was established that solid substrates with high phytate and low inorg. phosphate (Pi) contents are substrate of choice for phytase prodn. by SSF. [on SciFinder(R)]