Extraction and phytochemical evaluation of Litsea glutinosa bark methanolic extract

Abstract

The present investigation deals with the extraction of bark powder of Litsea glutinosa and its preliminary phytochemical screening. The bark powder was subjected to methanolic extraction and further explored for its phytochemical constituents using TLC and GC MS. Preliminary phytochemical screening reveals the presence of alkaloids, flavonoides, glycosides, phenols, tannins and saponins. The extract was further subjected to separation using TLC and fractions were evaluated using GC MS. GC MS analysis of the total methanolicextract showed the presence of Oliec acid, tricosene, erucic acid, tetra decanoic acid, pyrrolidinone, piperidine, eicosanoic acid like major phytochemicals. Alkaloid fraction was found to be rich in therapeutically potential compounds like Eicosane, Pieprizine, pyridine, thio-coumarin, tetrahydroisoquinoline. Apart from this various Androstane, Androsta-trione, pregnene like phytoestrogens were also observed in this plant, justifying its aphrodisiac and osteoprotective effect. TLC of various subfractions of alkaloids revealed that this plant is rich in variety of potential therapeutic phytochemicals, hence justifying its ethnomedical usage.