Bud burst timing in Picea abies seedlings as affected by temperature during dormancy induction and mild spells during chilling

Abstract

In trees adapted to cold climates, conditions during autumn and winter may influence the subsequent timing of bud burst and hence tree survival during early spring frosts. We tested the effects of two temperatures during dormancy induction and mild spells (MS) during chilling on the timing of bud burst in three Picea abies (L.) Karst. provenances (58-66° N). One-year-old seedlings were induced to become dormant at temperatures of 12 or 21 °C applied during 9 weeks of short days (12-h photoperiod). The seedlings were then moved to cold storage and given either continuous chilling at 0.7 °C (control), or chilling interrupted by one 14-day MS at either 8 or 12 °C. Interruptions with MS were staggered throughout the 175-day chilling period, resulting in 10 MS differing in date of onset. Subsets of seedlings were moved to forcing conditions (12-h photoperiod, 12 °C) throughout the chilling period, to assess dormancy status at different timings of the MS treatment. Finally, after 175 days of chilling, timing of bud burst was assessed in a 24-h photoperiod at 12 °C (control and MS-treated seedlings). The MS treatment did not significantly affect days to bud burst when given early (after 735 chilling days). When MS was given after 49 chilling days or later, the seedlings burst bud earlier than the controls, and the difference increased with increasing length of the chilling period given before the MS. The 12 °C MS treatment was more effective than the 8 °C MS treatment, and the difference remained constant after the seedlings had received 66 or more chilling days before the MS treatment was applied. In all provenances, a constant temperature of 21 °C during dormancy induction resulted in more dormant seedlings (delayed bud burst) than a constant temperature of 12 °C, but this did not delay the response to the MS treatment.