Purification of Encephalitozoon cultures contaminated by mycoplasmas by murine intraperitoneal inoculation

7Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Encephalitozoon species are strict intracellular microsporidia. Cocultures with eukaryotic cell lines can become accidently contaminated by mycoplasmas. We propose a decontamination protocol based on differential cell targeting after intraperitoneal inoculation in mice. Mycoplasma-free microsporidia were isolated from the brains and spleens of inoculated mice 24 h postinoculation by using the centrifugation shell vial system. Identification was confirmed by direct sequencing of PCR-amplified 16S rRNA.

Cite

CITATION STYLE

APA

Ridoux, O., Foucault, C., & Drancourt, M. (1998). Purification of Encephalitozoon cultures contaminated by mycoplasmas by murine intraperitoneal inoculation. Journal of Clinical Microbiology, 36(8), 2380–2382. https://doi.org/10.1128/jcm.36.8.2380-2382.1998

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free