Encephalitozoon species are strict intracellular microsporidia. Cocultures with eukaryotic cell lines can become accidently contaminated by mycoplasmas. We propose a decontamination protocol based on differential cell targeting after intraperitoneal inoculation in mice. Mycoplasma-free microsporidia were isolated from the brains and spleens of inoculated mice 24 h postinoculation by using the centrifugation shell vial system. Identification was confirmed by direct sequencing of PCR-amplified 16S rRNA.
CITATION STYLE
Ridoux, O., Foucault, C., & Drancourt, M. (1998). Purification of Encephalitozoon cultures contaminated by mycoplasmas by murine intraperitoneal inoculation. Journal of Clinical Microbiology, 36(8), 2380–2382. https://doi.org/10.1128/jcm.36.8.2380-2382.1998
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