Comparative study of BMP-2 gene delivery to Human adipose tissue-derived mesenchymal stem cells with Turbofect and Polyethylenimine

Abstract

Genetic modification of mesenchymal stem cells (MSCs) with plasmid encoding the bone morphogenetic protein 2 gene (BMP2) is a crucial task in the development of gene therapy and tissue engineering technologies for bone regeneration. The low transfection efficiency of MSC cultures is a limitation for practical applications and makes it necessary to search for optimal protocols that ensure efficient gene delivery while maintaining sufficient cell viability. Comparison of two transfecting reagents (TurboFect and Polyethylenimine) shows that TurboFect is the most effective for MSCs transfection. A higher level of target gene BMP2 expression and osteogenic differentiation can be achieved using the TaqRFP-N-BMP2 plasmid compared with pcDNA3-BMP2.