Extraction of bixin from annatto seeds with microwave

Abstract

This study aims to determine the effect of extraction time (5,10,15 minutes) and the ratio of the aquadest solvent: ethyl acetate (1: 6.5, 1: 7, 1: 8 v/v) to the yield of pigment. The optimum condition was found in the ratio of the solvent of distilled water: ethyl acetate (1: 7 v/v) extraction time of 15 minutes with low microwave power resulted in maximum pigment yield of 0.469%. The isolate compounds were analyzed using IR spectroscopy, mass spectroscopy, and High-Performance Liquid Chromatography (HPLC) showed that the isolates were boxing pigments having a molecular weight of 394 g/Mol with wavenumbers for OH groups, C = O carboxylate groups, CH bonds, duplicate C = C, and CO ester bonds. As well as showing the retention time of standard and standard pigments at minutes to 12.849 and 12.843.