Brachyury regulatory region active in embryonal carcinoma p19 cells

Abstract

Brachyury (T) is involved in mesoderm induction during early mouse development. We analyzed the region regulating expression of T in embryonal carcinoma P19 cells, which differentiate into mesoderm derivatives in vitro. Transfection of plasmids encoding reporter genes under the control of the 5′-flanking region showed positive regulatory elements between -298 and -129 bp are responsible for driving T expression in mesodermal cells. © 1999, Taylor & Francis Group, LLC. All rights reserved.