Characterization of a New Flavone and Tyrosinase Inhibition Constituents from the Twigs of Morus alba L

Abstract

The twigs of Morus alba L. were found to show strong tyrosinase inhibition activity, and the responsible active components in the extract were further investigated in this study. A flavone, named morusone (1), and sixteen known compounds 2-17 were isolated from M. alba twigs and their structures were identified by interpretation of the corresponding ESI-MS and NMR spectral data. In the tyrosinase inhibitory test, the compounds steppogenin (IC50 0.98 ± 0.01 μM), 2,4,2? ,4? -tetrahydroxychalcone (IC50 0.07 ± 0.02 μM), morachalcone A (IC50 0.08 ± 0.02 μM), oxyresveratrol (IC50 0.10 ± 0.01 μM), and moracin M (8.00 ± 0.22 μM) exhibited significant tyrosinase inhibition activities, much stronger than that of the positive control kojic acid. These results suggest that M. alba twig extract should served as a good source of natural tyrosinase inhibitors for use in foods as antibrowning agents or in cosmetics as skin-whitening agents.