Purified pyocyanin from clinical isolates of pseudomonas aeruginosa enhances antibiotic sensitivity against some pathogenic bacteria

Abstract

Pseudomonas aeruginosa is one of the most life-threatening pathogen. It is considered nosocomial opportunistic microbe that cause wide range of infections including wound and burn infections, respiratory infections, and Otitis media. Despite the efficiency of antibiotics against infectious diseases, P. aeruginosa still causes complicated infections with antibiotic resistance in many clinical strains. The pigments produced by P. aeruginosa exhibits antibacterial properties. Thus, we have examined its ability to enhance antibiotics effect against some pathogenic microbes. 286 samples were collected from patients with different infections who visited Mosul hospitals. 76 samples were positive to P.aeruginosa. Among them, 38 (13.28%) of isolates were isolated from surgical infection, whereas, 12 (4.19%), 11(3.84%), and 7(2.447%) were isolated from Otitis media, Urinary tract infection, and pus, respectively. The pyocyanin in low concentrations showed synergistic effect with some antibiotics against Staphylococcus aureus and Escherichia coli. E. coli became sensitive to ciprofloxacin and nalidixic acid when mixed with 100 mg/ml pyocyanin. However, cloxacillin did not show any activity against Staph.aureus when mixed with 1 mg/ml and 6.25 mg/ml pyocyanin. Staph aureus became sensitive to nalidixic acid when mixed with 1 mg/ml pyocyanin.