Efek perlindungan kombinasi kuersetin dan omega-3 terhadap sel β pankreas tikus dm tipe 2

Abstract

Diabetes mellitus (DM) is a metabolic disease that causes damage pancreatic cells. Quercetin and omega-3 widely studied as an adjunctive therapy in DM because it can reduce oxidative stress and prevent cell damage. The aim of the study was to measure the ability of quercetin and omega-3 combination in protecting pancreatic β-cells in diabetic rats. Subjects consisted of 28 rats, divided into 1 healthy group and 6 diabetic groups (4 rats per group). The tested materials are quercetin doses 5, 20 and 80 mg/kg/day and omega-3 doses 25, 100 and 400mg/kg /day orally for 4 weeks. After treatment, the pancreatic tissue was taken for hematoxylin-eosin staining and be calculated the percentage of it damage. In this research, combination of quercetin 80mg/kg/day and omega-3 400 mg /kg/day lowered levels of destructionof pancreatic β cells significantly better than a smaller dose combination or without the combination (p<0,05). So, combination of quercetin 80 mg/kg/day and omega-3 400 mg/kg/day lowered levels of destruction of pancreatic β-cells most significantly better than a smaller ose combination or without the combination.