Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae

Abstract

Correction to: Mol Syst Biol (2014) 10: 716. DOI 10.1002/msb.134766 | Published online 31 January 2014[OpenUrl][1][Abstract/FREE Full Text][2] We were recently made aware of an error in the equation that we used to estimate acetylation stoichiometry in the above‐mentioned article. While this error does not have a major impact on our results or their interpretation, some of the numbers are changed slightly and here we provide updated figures and tables, the equation used, and the revised stoichiometry estimates for individual sites. To calculate initial stoichiometry (SI), we previously divided the degree of partial chemical acetylation (C) by the SILAC ratio (R) of increased acetylation after partial chemical acetylation, SI = C/R. However, in the correct equation, the SILAC ratio should be subtracted by (1−C), SI = C/(R−(1−C)). This equation was derived as follows. If the final stoichiometry is (SF) = SI + C(1−SI) and the acetylated peptide SILAC ratio (R) = SF/SI, then solving the above equation for SI in terms of SF/SI yields SI = C/((SF/SI) −(1−C)) = C/(R−(1−C)). We determined that C was < 1%, and we estimated stoichiometry using the conservative assumption that C = 1% (0.01); therefore, we previously calculated stoichiometry using the equation S = 0.01/R, while the correct equation is S = 0.01/(R−0.99). We further realized that we could use a previously published equation (Olsen et al, 2010) for calculating stoichiometry and this equation yielded results identical to the revised formula presented above. We now include both equations and the updated stoichiometry estimates in Supplementary Table S9. The new equation had a very small impact on our stoichiometry estimates. Regardless, we … [1]: {openurl}?query=rft.jtitle%253DMolecular%2BSystems%2BBiology%26rft.stitle%253DMol%2BSyst%2BBiol%26rft.aulast%253DWeinert%26rft.auinit1%253DB.%2BT.%26rft.volume%253D10%26rft.issue%253D1%26rft.spage%253D716%26rft.epage%253D716%26rft.atitle%253DAcetylation%2Bdynamics%2Band%2Bstoichiometry%2Bin%2BSaccharomyces%2Bcerevisiae%26rft_id%253Dinfo%253Adoi%252F10.1002%252Fmsb.134766%26rft_id%253Dinfo%253Apmid%252F24489116%26rft.genre%253Darticle%26rft_val_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Ajournal%26ctx_ver%253DZ39.88-2004%26url_ver%253DZ39.88-2004%26url_ctx_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Actx [2]: /lookup/ijlink?linkType=ABST&journalCode=msb&resid=10/1/716&atom=%2Fmsb%2F11%2F10%2F833.atom