In vivo and in silico anti-arthritic studies of chlorogenic acid from the rhizome of Lasia spinosa (L.) Thwaites (Araceae)

Abstract

Aim: The aim of the study was to examine the anti-arthritic function of methanol extract of Lasia spinosa (MELS) and chlorogenic acid (CA) in the rats of Freund’s complete adjuvant (FCA) mediated arthritis by in vivo and In silico studies. Materials and Methods: The MELS was subjected to acute oral toxicity in rats and tested against FCA induced arthritis in rats. Large-scale isolation and chromatographical analysis with spectral review verified that CA is responsible for the pharmacological effects reported. The efficacy of MELS and CA against CFA-induced arthritis was subsequently tested for hematological, biochemical, and in vivo anti-oxidant parameters in rats on the last day of the research. The interleukin-6 (IL-6) and tumor necrosis factor-α expressions in the paw tissue were determined by Western blotting technique. Ankle joint histopathological and radiological studies were also conducted. Results: MELS and CA dosage based on anti-arthritis, which was obvious in comparison with an arthritis control group with decreased paw volume, joint diameter, and body weight. MELS and CA showed significant anti-arthritic activity by rising RBC and Hb levels and decreasing WBC and platelet levels. The anti-arthritis function has also been verified by the altered biochemical and anti-oxidant parameters MELS (400 and 200 mg/kg) and CA (5, 10 mg/kg). The activity was further confirmed by histopathological radiological tests with in silico analysis. Conclusion: This study confirms the ethnomedicinal use of L. spinosa rhizomes in the treatment of arthritis. It also indicates the strong anti-arthritic effects of CA. Additional clinical trials are, however, required to show the effectiveness of CA in different immuno-inflammatory conditions.