Studies on Sporopollenin Biosynthesis: The Effect of Inhibitors of Carotenoid Biosynthesis on Sporopollenin Accumulation

Abstract

1. The unequivocal chemical com position of sporopollenin is unknown. It was often hypothesized that sporopollenin represents a derivative of carotenoids and/or carotenoid esters. Proceeding from this working hypothesis the influence of inhibitors of carotenoid biosynthesis (DPA, nicotine, Sandoz) on the sporopollenin accumulation was studied in order to test whether an intact carotenoid m etabolism is involved. 2. Drastic changes in carotenoid spectra as well as incarotenoid patterns in pollen extracts were used as a ™marker™ for the uptake and transport of the inhibitors intothe anther loculus, the site of sporopollenin biosynthesis. Of the three inhibitors of carotenoid metabolism, D PA, nicotine and Sandoz, only Sandoz appeared to influence carotenoid metabolism in the anthers. 3. After application ofSandozthe sporopollenin accumulation in anthers and pollen was only slightly affected. The amount of sporopollenin after treatm ent is similar to, or possibly even marginally higher than the control, if dry weight is chosen as reference. If the amount is expressed with respect to one pollen grain, the content is very slightly decreased in the case of pollenfromtreated plants. Therefore, it is concluded that severe interference in carotenoid biosynthesis does not result in asubsequent drastic inhibition of sporopollenin biosynthesis. 4. A significant increase of PAL activity under the influence of Sandoz indicates that effects upon phenylpropanoid metabolism are possible. © 1985, Walter de Gruyter. All rights reserved.