Dimerization choices control the ability of axin and dishevelled to activate c-Jun N-terminal kinase/stress-activated protein kinase

Abstract

Axin and Dishevelled are two downstream components of the Wnt signaling pathway. Dishevelled is a positive regulator and is placed genetically between Frizzled and glycogen synthase kinase-3β, whereas Axin is a negative regulator that acts downstream of glycogen synthase kinase-3β. It is intriguing that they each can activate the c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) when expressed in the cell. We set out to address if Axin and Dishevelled are functionally cooperative, antagonistic, or entirely independent, in terms of the JNK activation event. We found that in contrast to Axin, Dv12 activation of JNK does not require MEKK1, and complex formation between Dv12 and Axin is independent of Axin-MEKK1 binding. Furthermore, Dv12-DIX and Dv12-ΔDEP proteins deficient for JNK activation can attenuate Axin-activated JNK activity by disrupting Axin dimerization. However, Axin-ΔMID, Axin-ΔC, and Axin-CT proteins deficient for JNK activation cannot interfere with Dv12-activated JNK activity. These results indicate that unlike the strict requirement of homodimerization for Axin function, Dv12 can activate JNK either as a monomer or homodimer/heterodimer. We suggest that there may be a switch mechanism based on dimerization combinations, that commands cells to activate Wnt signaling or JNK activation, and to turn on specific activators of JNK in response to various environmental cues.