Synthesis of Triphala Incorporated Zinc Oxide Nanoparticles and Assessment of its Antimicrobial Activity Against Oral Pathogens : An In-Vitro Study

Abstract

Triphala, a traditional Ayurveda herbal formulation consisting of dried fruits of three medicinal plants, is known to have effective antimicrobial properties. Inorganic elements like zinc oxide have also been known to have antimicrobial properties against strains of gram positive and gram negative bacteria, both. Green synthesis of nanoparticles is being encouraged, because of its positive effects on the environment and its cost-effectiveness. Hence, the study was conducted to synthesize Triphala augmented Zinc oxide nanoparticles and assess its antimicrobial activity against oral pathogens. Aqueous extract of Triphala was formulated and incorporated to zinc oxide solution to formulate the nanoparticle. Agar well diffusion method was used to assess the antimicrobial activity against S. Mutans, C. albicans, S. aureus, E.faecalis. Triphala incorporated zinc oxide nanoparticles produced a zone of inhibition more than that of ampicillin/cycloheximide against S. aureus and at 50 mu l was almost as effective as antibiotic against the gram positive bacteria. Triphala incorporated ZnONPs as a cost effective, eco-friendly yet efficient method and they show great potential as oral antimicrobial agents.