Somatic embryogenesis induction in Narcissuspapyraceus cv. Shirazi

Abstract

In order to investigate somatic embryogenesis in daffodil (Narcissus papyraceus cv. Shirazi) several experiments were carried. Explants were excised from the bulb scales and were transferred to MS and Nitsch media containing different concentrations of growth regulators such as BAP, 2,4-D, GA 3, Kin, IBA and NAA. The results showed that the highest number of regenerated bulblets were produced in Nitsch medium containing BAP (2.2 mg L-1) and 2,4-D (1.1 mg L-1). The highest rate of direct somatic embryos were observed on MS medium containing GA3 (0.5 mg L-1), BAP (1.6 mg L-1) and 2,4-D (1.6 mg L-1). To propagate the induced somatic embryos, they were transferred to hormone free MS medium. Then the produced bulblets were transferred to half MS medium containing IBA (1 mg L-1) for root induction. © 2007 Asian Network for Scientific Information.