Imaging translation in single cells using fluorescent microscopy

32Citations
Citations of this article
190Readers
Mendeley users who have this article in their library.

Abstract

The regulation of translation provides a mechanism to control not only the abundance of proteins, but also the precise time and subcellular location that they are synthesized. Much of what is known concerning the molecular basis for translational control has been gleaned from experiments (e.g., luciferase assays and polysome analysis) that measure average changes in the protein synthesis of a population of cells, however, mechanistic insights can be obscured in ensemble measurements. The development of fluorescent microscopy techniques and reagents has allowed translation to be studied within its cellular context. Here we highlight recent methodologies that can be used to study global changes in protein synthesis or regulation of specific mRNAs in single cells. Imaging of translation has provided direct evidence for local translation of mRNAs at synapses in neurons and will become an important tool for studying translational control. © 2012 Cold Spring Harbor Laboratory Press; all rights reserved.

Cite

CITATION STYLE

APA

Chao, J. A., Yoon, Y. J., & Singer, R. H. (2012). Imaging translation in single cells using fluorescent microscopy. Cold Spring Harbor Perspectives in Biology, 4(11). https://doi.org/10.1101/cshperspect.a012310

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free