Isolation and characterization of listeria monocytogenes bacteriophages from environmental sources in Kars-Turkey

Abstract

In this study, 45 samples were collected from some commercial businesses and environmental focus located in Kars center. Dirty areas, the area in contact with the sewage and poultry environmental sources were preferred while collecting samples. Again, 3 different silage samples and two samples were suspicious of Listeria were evaluated that brought to Kafkas University, Faculty of Veterinary Microbiology Laboratory. Isolation of lytic phages and lysogenic phages with mitomycin-C from total of 50 samples was performed. From 1 out of 12 of these samples lysogenic, from the remaining 11 samples lytic phage isolation was made. Isolation was confirmed by plaque assay. RAPD-PCR method was used for genotyping. The agarose gel images of 3 phages were observed to have been determined as previous study vB_SepiS-philPLA7, vB_SauS-philPLA35 and ΦH5. When band profiles belong to remaining 9 phages were compared, band profiles of 3 phages and the other 5 phages band profiles evaluated to be the same with each other. This study is thought to be precursors for purposes such as the decontamination of silage will be produced in the region and animal food, since Listeria infections are important for animal and human health and cause considerable economic losses.