Extraction of fast changes in the structure of a disordered ensemble of photoexcited biomolecules

Abstract

Using pump-probe experiments of varying time intervals between pump and probe, the method of time-resolved crystallography has given many insights into the fast time variations of crystallized molecules as a result of photoexcitation. We show here that quantities extractable from multiple diffraction patterns of dissolved molecules in random orientations, as measured using powerful ultrashort pulses of X-rays, also contain information about structural changes of a molecule on photoexcitation. © 2013 H.-C. Poon et al.