Mangrovimonas spongiae sp. nov., a novel member of the genus mangrovimonas isolated from marine sponge

Abstract

A taxonomic study was carried out on strain HN-E26T, which was isolated from sponge collected from Yangpu Bay, Hainan, PR China. Cells of strain HN-E26T were Gram-stain-negative, motile by gliding, yellow-pigmented and rod-shaped. The strain could grow at 10–40 °C (optimum, 25 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in 0.5–12% (w/v) NaCl (optimum, 4–7%). This isolate was positive for oxidase, catalase, and the hydrolysis of starch, xylan, aesculin and gelatin, but negative for indole production and the reduction of nitrate. Strain HN-E26T shared the highest 16S rRNA gene sequence similarity with Mangrovimonas yunxiaonensis LYYY01T (95.5%), followed by Formosa spongicola A2T (94.4%), Meridianimaribacter flavus NH57NT (94.3%) and Winogradskyella exilis 022-2-26T (94.3%). The phylogenetic tree based on 16S rRNA gene sequences revealed that strain HN-E26T formed a distinct phylogenetic lineage within the cluster comprising Mangrovimonas yunxiaonensis LYYY01T and ‘Mangrovimonas xylaniphaga’ ST2L12T. The dominant fatty acids were iso-C15:0 and iso-C15:1 G. The major polar lipids comprised phosphatidylethanolamine, three unidentified aminolipids and six unidentified lipids. The respiratory lipoquinone was identified as MK-6. The G+C content of the genomic DNA was 33.9 mol%. Based on the phenotypic and phylogenetic data, strain HN-E26T represents a novel species of the genus Mangrovimonas, for which the name Mangrovimonas spongiae sp. nov. is proposed, with the type strain HN-E26T (=MCCC 1K03326T=LMG 30458T).