The effects of inbreeding and heat stress on male sterility in Drosophila melanogaster

Abstract

Understanding the consequences of inbreeding in combination with stress is important for the persistence of small endangered populations in a changing environment. Inbreeding and stress can influence the population at all stages of the life cycle, and in the last two decades a number of studies have demonstrated inbreeding depression for most life-cycle components, both in laboratory populations and in the wild. Although male fertility is known to be sensitive to temperature extremes, few studies have focused on this life-cycle component. We studied the effects of inbreeding on male sterility in benign and stressful environments using Drosophila melanogaster as a model organism. Male sterility was compared in 21 inbred lines and five non-inbred control lines at 25.0 and 29.0°C. The effect of inbreeding on sterility was significant only at 29.0°C. This stress-induced increase in sterility indicates an interaction between the effects of inbreeding and high-temperature stress on male sterility. In addition, the stress-induced temporary and permanent sterility showed significant positive correlation, as did stress-induced sterility and the decrease in egg-to-adult viability. This suggests that the observed stress-induced decline in fitness could result from conditionally expressed, recessive deleterious alleles affecting both sterility and viability simultaneously. © 2011 The Linnean Society of London.