Optimization Method Based on Visualization of Sensitivity and Separation Performance in HPLC

Abstract

In UHPLC (Ultra High Performance Liquid Chromatography), by simultaneously representing the speed and separation performance in a graph, a reciprocal relationship has been elucidated. Although UHPLC is said to contribute to high-sensitivity performance, its theoretical formulation and visualization have not progressed. In this article, it was formulated considering that the high solute concentration in the flow cell of the UV detector after separation has a low detection limit and desirable performance, that is, high sensitivity. As a result, it was understood that there are effects peculiar to UHPLC regarding pressure and particle diameter, in addition to the effect of reducing the column cross-sectional areas as represented by semi-micro LC. In addition, it was found that the separation performance and the sensitivity performance conflict with each other by introducing the performance characteristic Σ called height-length product and displaying Σ on a three-dimensional graph.