Erratum: Glutamate dehydrogenase (Gdh2)-dependent alkalization is dispensable for escape from macrophages and virulence of Candida albicans (PLoS Pathogens (2020) 16:9 (e1008328) DOI: 10.1371/journal.ppat.1008328)

Abstract

After this article [1] was published, we discovered that the results reported for Gdh2-GFP in Figs 2–4 instead represented results from a strain expressing Put2-GFP. This error was discovered when validating a novel set of strains individually carrying GFP-tagged mitochondrial proteins. Strain CFG273 (GDH2/GDH2-GFP) as published, was used as a positive control. To our surprise and dismay, strain CFG219, constructed during the same period as CFG273, expressed a Put2-GFP protein that migrated precisely as the GFP-tagged construct expressed in CFG273. Subsequent Mass Spec analysis of immunoprecipitated GFP constructs (GFP-pull-downs) confirmed that the presumed Gdh2-GFP in CFG273 was in fact Put2-GFP. We traced this error to the freezing of strains, i.e., CFG219 was frozen twice, once as CFG273. The original CFG273 strain carrying a PCR-verified GDH2-GFP allele was thereby lost. Since identifying this error, we reconstructed CFG273 using the purified GDH2-GFP tagging cassette that was originally used and fully verified the GDH2-GFP allele precisely as described (see the updated S1 Fig of this notice) and repeated the localization and expression experiments. Data confirming correct targeting and identification of the resultant strain are in S1 Fig of this notice. We provide here updated versions of Figs 2–4, S1 Vid and S2 Vid, that report results obtained using the correct Gdh2-GFP strain. For comparison, the updated figures and videos also retain the results, now correctly attributed to Put2-GFP. The results regarding Put2 have not previously been published and are of additive value to understanding proline metabolism in C. albicans.