Association of a single nucleotide polymorphism in the bovine leptin gene with feed intake, feed efficiency, growth, feeding behaviour, carcass quality and body composition

Abstract

Leptin is a 16-kDa-hormone product of the obese gene synthesized and expressed predominantly by adipose tissues, which has been shown to play major roles in the regulation of body weight, feed intake, energy balance, fertility, and immune system functions. We report an investigation into the association of a previously identified cytosine to thymine missense mutation in exon 2 of the bovine leptin gene with feed intake, feed efficiency, growth, feeding behaviour, carcass quality and body composition in five genetic selection lines of a commercial population of beef cattle. Differences among genotypes in growth, feed intake and feed efficiency were not significant (P > 0.10) though steers homozygous for the thymine allele had positive residual feed intake (i.e., consumed more feed than expected) (+ 0.19 kg d-1) whilst steers homozygous for the cytosine allele had negative residual feed intake (-0.18 kg d-1). Steers carrying the thymine allele had a higher rate of gain in ultrasound backfat (P = 0.02), ultrasound backfat thickness (P = 0.06), higher carcass grade fat (backfat) (P = 0.005), lower yield grade (P = 0.01) and lower lean meat yield (P = 0.007). The thymine allele also tended to be associated with higher loin subcutaneous fat (P = 0.07) and was associated with higher brisket subcutaneous fat (P = 0.01), and brisket body cavity fat (P = 0.0001). No associations were found among the different genotypes and real-time ultrasound marbling, ultrasound longissimus thoracis area, carcass marbling and carcass longissimus thoracis area (P > 0.10). Our results show that animals carrying the thymine allele versus the cytosine allele may produce carcasses with poorer grades and lower lean meat yields but do not differ in carcass marbling or other fat depots.