Structure and mode of action of the membrane-permeabilizing antimicrobial peptide pheromone plantaricin A

Abstract

The three-dimensional structure in dodecyl phosphocholine micelles of the 26-mer membrane-permeabilizing bacteriocin-like pheromone plantaricin A (PlnA) has been determined by use of nuclear magnetic resonance spectroscopy. The peptide was unstructured in water but became partly structured upon exposure to micelles. An amphiphilic α-helix stretching from residue 12 to 21 (possibly also including residues 22 and 23) was then formed in the C-terminal part of the peptide, whereas the N-terminal part remained largely unstructured. PlnA exerted its membrane-permeabilizing antimicrobial activity through a nonchiral interaction with the target cell membrane because the D-enantiomeric form had the same activity as the natural L-form. This nonchiral interaction involved the amphiphilic α-helical region in the C-terminal half of PlnA because a 17-mer fragment that contains the amphiphilic α-helical part of the peptide had antimicrobial potency that was similar to that of the L- and D-enantiomeric forms of PlnA. Also the pheromone activity of PlnA depended on this nonchiral interaction because both the L- and D-enantiomeric forms of the 17-mer fragment inhibited the pheromone activity. The pheromone activity also involved, however, a chiral interaction between the N-terminal part of PlnA and its receptor because high concentrations of the L-form (but not the D-form) of a 5-mer fragment derived from the N-terminal part of PlnA had pheromone activity. The results thus reveal a novel mechanism whereby peptide pheromones such as PlnA may function. An initial nonchiral interaction with membrane lipids induces α-helical structuring in a segment of the peptide pheromone. The peptide becomes thereby sufficiently structured and properly positioned in the membrane interface, thus enabling it to engage in a chiral interaction with its receptor in or near the membrane water interface. This membrane-interacting mode of action explains why some peptide pheromones/hormones such as PlnA sometimes display antimicrobial activity in addition to their pheromone activity. © 2005 by The American Society for Biochemistry and Molecular Biology, Inc.