Corrective measures of denaturing gradient gel electrophoresis limitations

Abstract

Denaturing Gradient Gel Electrophoresis (DGGE) is a technique that attempts to monitor microbial populations, without culture dependant methods. This molecular biology approach is a fingerprinting methodology that revolutionary changes many of the traditional routines used in assessing microbial populations, PCR-DGGE is classified as part of the new discipline of molecular microbial ecology. Therefore, this study will briefly explain a number of applications that involve the technique and will also examine how DGGE works in general. In this study, various limitations of the DGGE technique and the corrective measures were briefly described that can improve the profitability, to get a real biodiversity.