Toxicity test of stem bark extract of banyuru (Pterospermum celebicum miq.) using BSLT (brine shrimp lethality test) and cream irritation test

Abstract

A study to observe toxic effect of ethanol extract of Pterospermum celebicum Miq. by using Brine Shrimp Lethality Test (BSLT) has been carried out. The method to do this study was irritation test of cream formula of the extract. The aim of this study was to collect data about toxicity of fractionated extract of P. celebicum Miq. on Artemia salina by using BSLT method according to LC50 value obtained from probit analysis. The extract was extracted by maceration method using ethanol as solvent which was then formulated as cream containing 3% of P. celebicum Miq. extract. The toxicity test revealed that the value of LC50 was 15.15 g/mL, while irritation test stated that no erythema and edema observed.