Compensatory responses after unilateral ovariectomy in rabbits.

Abstract

Compensatory ovarian and gonadotropic responses to unilateral ovariectomy (ULO) were examined in the rabbit doe, an induced ovulator. On Days 2, 4, 5, 6, 8, 10, 15 and 20 after ULO, ovaries from 3 hemiovariectomized does and 1 sham-hemiovariectomized doe were examined macro- and microscopically for number, size and signs of atresia of follicles. The number of surface follicles increased initially to 7 or 8 follicles 2 days after ULO, followed by an increase to 10 or more follicles by Day 15 (control ovaries had 5.7 +/- 0.4 follicles). Total numbers of antral follicles and the proportion of follicles which were atretic did not vary relative to day after ULO. However, distributions of antral follicles in classes of 0.2-mm increments were significantly different between sham-ovariectomized and hemiovariectomized does after Day 2 due to shifts of follicles into larger size classes. Peripheral serum concentrations of follicle-stimulating hormone (FSH), but not luteinizing hormone (LH), increased temporarily during the 48 h after ULO. Follicular compensation after ULO in the doe entailed nonlinear increases in numbers of preovulatory follicles, due to increased growth within the antral population of follicles, probably the result of an acute surge of FSH. A period of more than 10 days was necessary to restore the number of preovulatory follicles after ULO. Exogenous human chorionic gonadotropin (hCG) induced ovulation of recruited follicles.