Production of an extracellular polyethylene-degrading enzyme(s) by Streptomyces species

Abstract

Extracellular culture concentrates were prepared from Streptomyces viridosporus T7A, Streptomyces badius 252, and Streptomyces setonii 75Vi2 shake flask cultures. Ten-day-heat-treated (70°C) starch-polyethylene degradable plastic films were incubated with shaking with active or inactive enzyme for 3 weeks (37°C). Active enzyme illustrated changes in the films' Fourier transform infrared spectra, mechanical properties, and polyethylene molecular weight distributions.