Association of a polymorphism of BCHE with ischemic stroke in Japanese individuals with chronic kidney disease

Abstract

Although chronic kidney disease (CKD) is an important risk factor for ischemic stroke, the genetic variants that confer susceptibility to ischemic stroke in individuals with CKD remain largely unknown. We performed an association study for candidate gene polymorphisms and ischemic stroke in individuals with CKD. The study population comprised 1041 Japanese individuals with CKD, including 228 subjects with ischemic stroke and 813 controls. The genotypes of 150 polymorphisms of 127 candidate genes were determined by a method that combines polymerase chain reaction and sequence-specific oligonucleotide probes with suspension array technology. An initial χ2 test (false discovery rate <0.05) and subsequent multivariate logistic regression analysis with adjustment for covariates (P<0.05) revealed that the 1615G→A (Ala539Thr) polymorphism (rs1803274) of BCHE (OR=3.33; 95% CI 1.32-8.28) and the 2445G →A (Ala54Thr) polymorphism (rs1799883) of FABP2 (OR=1.66; 95% CI 1.01-2.70) were significantly associated with ischemic stroke. The variant alleles of BCHE and FABP2 were risk factors for ischemic stroke. A stepwise forward selection procedure demonstrated that the BCHE genotype was a significant (P<0.05) and independent determinant of ischemic stroke. Genotyping for BCHE may prove informative for the assessment of the genetic risk of ischemic stroke in Japanese individuals with CKD.