CanID-PCR: a quick and low-cost PCR tool to identify Candida species on gDNA directly extracted from positive blood bottles

Abstract

Identifying species in Candida infections is of utmost importance to clinicians and researchers alike. Here, we describe CanID-PCR: a quick and low-cost PCR tool to identify Candida species on genomic DNA directly extracted from positive blood cultures. This technique uses basic laboratory equipment, and there is no need for high investment in special equipment. The tool allows identification of the nine most prevalent species in candidiasis. The technique is simple and can be used without the need for an additional culture to isolate the cells. It is noteworthy to mention that this tool can detect polymicrobial infections caused by more than one Candida species.