Microbial biomass and organic matter in an oxisol under application of biochar

Abstract

The aim of this study was to investigate the short- and medium-term effect of biochar on soil microbial properties, organic carbon and nitrogen in an Oxisol from Brazil. The experiment was conducted in a randomized block design consisting of five levels of biochar (0, 2, 4, 8 and 16 Mg·ha-1) with and without of synthetic fertilizer: 0 and 200 kg.ha-1 of synthetic fertilizer N-P-K (00-20-20). The following soil properties were determined: microbial biomass carbon (MBC) and nitrogen (MBN), microbial respiration (MR), metabolic quotient (qCO2) and microbial quotient (qMIC), total organic carbon (TOC) and nitrogen (TN). The MBC, qCO2 and carbon management index (CMI) were not altered by doses of fertilizer and biochar. The presence of biochar reduced the MBN by 11% and 5% with the application of 16 Mg·ha-1 compared to control in the third and sixth year, respectively. The qMIC was reduced exponentially with the application of biochar, but was within normal limits as a proportion of total organic carbon. There was an increase in TOC and TN with the application of biochar. The use of biochar did not cause significant negative changes in soil microbial properties or contribute to carbon sequestration in the soil.