Isorhamnetin inhibits IL-1β-induced expression of inflammatory mediators in human chondrocytes

Abstract

Isorhamnetin (ISH) is a flavonoid primarily obtained from the fruit of Hippophae rhamnoides L., which possesses anti-inflammatory properties. However, the effect of ISH on the expression of inflammatory mediators in response to interleukin (IL)-1β stimulation has not been elucidated. The present study investigated the effects of ISH on the expression of inflammatory mediators in human chondrocytes, induced by IL-1β. The results of the present study demonstrated that pretreatment with ISH inhibited the expression of stromelysin-1 and collagenase 3 in chondrocytes, induced by IL-1β. Pretreatment with ISH inhibited the IL-1β-stimulated synthesis of NO and prostaglandin E2 induced by IL-1β, in addition to the expression of inducible nitric oxide synthase and prostaglandin G/H synthase 2 in chondrocytes. Additionally, ISH inhibited the expression of nuclear factor (NF)-κB and transcription factor p65, and the degradation of NF-κB inhibitor α induced by IL-1β in chondrocytes. In conclusion, the results of the present study indicated that ISH exhibited anti-inflammatory and chondroprotective effects in IL-1β-stimulated chondrocytes. The results of the present study suggest that ISH may be a potential agent in the future treatment of osteoarthritis.