Formulation of Quercetin Mouthwash and Anti-microbial Potential Against Critical Pathogens: An In-Vitro Evaluation

Abstract

Introduction Herbal mouthwashes were designed and prepared using essential oils from phytotherapeutic plants, containing active agents such as catechins, tannins, and sterols. Quercetin is one of the most abundant natural flavonoids predominantly found in foods including fruits, and vegetables. It has wide biological effects such as antioxidant, anti-aging, anti-inflammatory, probiotic, and metabolic modulation activities. Research has pointed toward its anti-microbial efficacy and bio-compatibility. Materials and methods A sample of commercially available Himalaya Hiora mouthwash (control) and 0.02 mg of Quercetin (test sample) that is sold commercially were procured. Next, 0.01 grams of sodium lauryl sulfate (SLS), which serves as the foaming agent, 0.001 grams of preservative (sodium methylparaben), and 0.3 grams of sucrose, the sweetening agent, were added to the test sample to formulate the mouthwash. We added 0.5 milliliters of the prepared Quercetin solution to the mixture. The effectiveness of the Quercetin mouthwash formulation as an antimicrobial was evaluated using the Agar-Well Diffusion Method against five oral pathogens and compared to the Hiora mouthwash. Results On quantifying the zone of inhibition, it was observed that at 100 μL of concentration, Staphylococcus aureus shows maximum inhibition rate, i.e., 15 mm when compared to commercially available herbal mouthwash (Himalaya Hiora). Thus, from the results obtained, we found that when concentration increases there is a significant zone of inhibition shown by oral pathogens. Conclusion Quercetin mouthwash formulation has proven to have a good antimicrobial effect when compared to standard mouthwash. The effective antimicrobial activity suggests its potential use as an adjuvant chemical plaque control modality. Further clinical trials would pave the way for its use as a routine or therapeutic antimicrobial agent.