Identification and characterization of amentoflavone from six species of Juniperus against H2O2 induced oxidative damage in human erythrocytes and leucocytes

Abstract

The aim of the present study was to investigate the protective effects of amentoflavone (AF), isolated from six species of Juniperus (AF1-AF6) (Cupressaceae) Juniperus communis L. (AF1), Juniperus wallichiana L. (AF2), Juniperus indica L. (AF3), Juniperus macropoda L. (AF4), juniperus recurva L. (AF5) and Juniperus polycarpos L. (AF6)] against H2O2 induced oxidative damage in human erythrocytes and leucocytes. The experiment was set and designed as per method available in literature. Amentoflavone was isolated from aerial parts of Juniperus species (above six) and authenticated by TLC, IR, HPLC and physicochemical parameters. The two concentration of isolated AF (A = 250 μL, B = 500 μL) were used to assessed its protective effects against H2O2 induced oxidative damage in human erythrocytes and leucocytes. The measurements of different antioxidant parameters like, CAT, SOD and GPx, LPO and GSH levels were performed under each set extracts. Juniperus communis L. (AF1B) (500 μL) and Juniperus indica L. (AF3B) (500 μL) were the most significant one on CAT (28.35±2.980 and 27.73±1.580 AU g-1 Hb), GPx (128.53±3.10 and 41.7±1.420 μg g-1 Hb) and SOD (1193.5±19.7 and 1532.6±17.60 U g-1 Hb) enzyme systems of erythrocytes. Among plant extracts, Juniperus communis L. (AF1B) showed the highest activities on CAT (13.79±1.832 AU mg-1 protein) and SOD (820±14.50 U mg-1 protein) while Juniperus indica L. (AF3B) showed significant effects on Gpx (152.72±3.70 U mg-1 protein), GSH (25.93±1.560 μg mg-1 protein) and LPO (10.54±2.90 nmol mg-1 protein) enzyme systems of leucocytes. The study concludes that isolated fractions of AF from Juniperus species (among six species), has a potential source of natural antioxidants for treatment and prevention of diseases in which oxidative stress takes place.